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  • Title: Label-free fluorescent aptasensor for potassium ion using structure-switching aptamers and berberine.
    Author: Guo Y, Chen Y, Wei Y, Li H, Dong C.
    Journal: Spectrochim Acta A Mol Biomol Spectrosc; 2015 Feb 05; 136 Pt C():1635-41. PubMed ID: 25459726.
    Abstract:
    A simple, rapid and label-free fluorescent aptasensor was fabricated for the detection of potassium ion (K(+) ion) in aqueous solution using K(+) ion-stabilized single stranded DNA (ssDNA) with G-rich sequence as the recognition element and a fluorescent dye, berberine, as the fluorescence probe. In the presence of K(+) ion, the G-rich ssDNA is promoted to form the aptamer-target complex with a G-quadruplex conformation, and berberine binding to the G-quadruplex structure results in the enhancement of its fluorescence. The fluorescence intensity of the sensing system displayed a calibration response for K(+) ion in the range of 0-1600μM with a detection limit of 31nM (S/N=3) and a relative standard deviation (RSD) of 0.45%. This label-free fluorescence aptasensor is conveniently and effectively applicable for analysis of K(+) ion in blood serum samples with the recovery range of 81.7-105.3%. The assay for detection of potassium ion is easy, economical, robust, and stable in rough conditions.
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