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  • Title: Enzymatic hydrolysis of steam-exploded sugarcane bagasse using high total solids and low enzyme loadings.
    Author: Ramos LP, da Silva L, Ballem AC, Pitarelo AP, Chiarello LM, Silveira MH.
    Journal: Bioresour Technol; 2015 Jan; 175():195-202. PubMed ID: 25459822.
    Abstract:
    Hydrolysis of phosphoric acid-impregnated steam-treated sugarcane bagasse was pre-optimized using a face-centered central composite design in which the process variables were the substrate total solids (TS, %), agitation intensity (AI, rpm) and enzyme loading (EL, gg(-1)). Pretreatment was carried out at 180°C for 10min using cane bagasse with 50wt% moisture content containing 9.5mg of H3PO4 per gram of dry biomass. Hydrolyses were performed for 96h at 50°C using Cellic CTec2® and water-washed steam-treated substrates. The highest amount of fermentable sugars was obtained with 20wt% TS, producing 76.8gL(-1) of glucose equivalents, which corresponded to a total glucan conversion of 69.2wt% and to a theoretical net increase of 39% in ethanol production from the same sugarcane tonnage without considering the use of leaves, tops and the additional yields from C5 sugars.
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