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  • Title: Purification of the mitochondrial carnitine carrier by chromatography on hydroxyapatite and celite.
    Author: Indiveri C, Palmieri F.
    Journal: FEBS Lett; 1989 Aug 14; 253(1-2):217-20. PubMed ID: 2547656.
    Abstract:
    The carnitine carrier from rat liver mitochondria has been extracted with Triton X-100 ad partially purified by chromatography on hydroxyapatite and celite. During purification the activity of the carrier was monitored by functional reconstitution into liposomes. The purified fraction is 250-fold enriched with respect to the N-ethylmaleimide-sensitive carnitine/carnitine transport activity. The substrate specificity and the inhibitor sensitivity of carnitine transport in liposomes resemble closely those described for the transport of carnitine in mitochondria.
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