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  • Title: Determination of rotavirus serotype-specific antibodies in sera by competitive enhanced enzyme immunoassay.
    Author: Beards GM, Desselberger U.
    Journal: J Virol Methods; 1989; 24(1-2):103-10. PubMed ID: 2547808.
    Abstract:
    A method is described for the specific detection of antibody to individual rotavirus serotypes in sera. A competitive enzyme immunoassay (EIA) was developed in which rotavirus serotype-specific monoclonal antibodies against VP7 compete with antibodies in test sera for rotavirus serotype-specific antigen bound to a solid phase. There was an excellent correlation between serotype-specific EIA results and serotype-specific neutralization titres (r = 0.915, P = less than 0.001). The value of this method for rotavirus epidemiology and vaccine trials is discussed.
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