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  • Title: Preparation and characterization of monomers to tetramers of a collagen-like domain from Streptococcus pyogenes.
    Author: Peng YY, Stoichevska V, Howell L, Madsen S, Werkmeister JA, Dumsday GJ, Ramshaw JA.
    Journal: Bioengineered; 2014; 5(6):378-85. PubMed ID: 25482084.
    Abstract:
    The collagen like domain Scl2 from Streptococcus pyogenes has been proposed as a potential biomedical material. It is non-cytotoxic and non-immunogenic and can be prepared in good yield in fermentation. The Scl2 collagen domain is about a quarter of the length, 234 residues, of the main collagen type, mammalian type I collagen (1014 residues) that is currently used in biomedical devices. In the present study we have made constructs comprising 1 to 4 copies of the Scl2 collagen domain, plus these same constructs with a CysCys sequence at the C-terminal, analogous to that found in mammalian type III collagens. The yields of these constructs were examined from 2 L fermentation studies. The yields of both series declined with increasing size. Circular dichroism showed that the addition of further collagen domains did not lead to a change in the melting temperature compared to the monomer domain. Addition of the CysCys sequence led to a small additional stabilization of about 2-3°C for the monomer construct when the folding (V) domain was present.
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