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  • Title: Crystallization and preliminary X-ray study of biosynthetic alanine racemase from Pseudomonas aeruginosa PAO1.
    Author: Zhou H, Li Z, Zhang G, Xu S, Tang Z, Zhu X, Dong H, Ju J.
    Journal: Acta Crystallogr F Struct Biol Commun; 2014 Dec 01; 70(Pt 12):1616-9. PubMed ID: 25484210.
    Abstract:
    Biosynthetic alanine racemase (AlrPA) from Pseudomonas aeruginosa PAO1 carrying a His6 tag was expressed in Escherichia coli BL21 (DE3) cells and purified by Ni(2+)-chelating affinity and anion-exchange chromatography for X-ray crystallographic analysis. Crystals were grown by the hanging-drop vapour-diffusion method at 289 K in a solution consisting of 4%(v/v) Tacsimate pH 5.0, 14%(w/v) polyethylene glycol 3350 with a protein concentration of 8 mg ml(-1). The crystal diffracted to 2.76 Å resolution and belonged to the orthorhombic space group P212121, with unit-cell parameters a = 74.12, b = 76.97, c = 154.80 Å, α = β = γ = 90°.
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