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  • Title: Achieving direct electrochemistry of glucose oxidase by one step electrochemical reduction of graphene oxide and its use in glucose sensing.
    Author: Shamsipur M, Tabrizi MA.
    Journal: Mater Sci Eng C Mater Biol Appl; 2014 Dec; 45():103-8. PubMed ID: 25491807.
    Abstract:
    In this paper, the direct electrochemistry of glucose oxidase (GOD) was accomplished at a glassy carbon electrode modified with electrochemically reduced graphene oxide/sodium dodecyl sulfate (GCE/ERGO/SDS). A pair of reversible peaks is exhibited on GCE/ERGO/SDS/GOD by cyclic voltammetry. The peak-to-peak potential separation of immobilized GOD is 28 mV in 0.1 M phosphate buffer solution (pH7.0) with a scan rate of 50 mV/s. The average surface coverage is 2.62×10(-10) mol cm(-2). The resulting biosensor exhibited a good response to glucose with linear range from 1 to 8 mM (R(2)=0.9878), good reproducibility and detection limit of 40.8 μM. The results from the biosensor were similar (±5%) to those obtained from the clinical analyzer.
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