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  • Title: DNA biosensor based on hybridization refractory mutation system approach for single mismatch detection.
    Author: Joda H, Beni V, Katakis I, O'Sullivan CK.
    Journal: Anal Biochem; 2015 Apr 01; 474():66-8. PubMed ID: 25524618.
    Abstract:
    We report on a simple approach to enhance solid-phase hybridization-based single base mismatch discrimination at high ionic strength based on the deliberate insertion of a natural DNA base mismatch in the surface-tethered probe. A large drop in hybridization signal of single base mismatched alleles using the designed probe as compared with the conventional probe, from 80% to less than 25% of the signal obtained with the fully complementary, non-mutation-containing sequence, when using colorimetric detection was further improved to 20% when using electrochemical detection, attributable to a difference of spacing of immobilized probes. Finally, the designed probe was used for the electrochemical detection of the DQA1*05:05 allele amplified from real human blood samples.
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