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  • Title: Wall effects in sucrose density gradient centrifugation of viruses.
    Author: Boeyé A, De Rees A.
    Journal: Arch Virol; 1989; 107(1-2):77-84. PubMed ID: 2552963.
    Abstract:
    The depletion of sedimenting particles to be expected from wall effects in the course of sucrose gradient centrifugation, was computed for various types of swing-out rotors on the assumption that particles hitting the tube wall are lost from the gradient. This assumption was roughly verified for heat-denatured, but not for native poliovirus. Tween 20 reduced the depletion, and prevented tight sticking of heat-denatured virus particles to tube walls.
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