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  • Title: Production of a fragment of glycoprotein G of herpes simplex virus type 2 and evaluation of its diagnostic potential.
    Author: Liu T, Liu JF, Yu H, Si GJ, Hu J, Li J.
    Journal: Singapore Med J; 2015 Jun; 56(6):346-52. PubMed ID: 25532518.
    Abstract:
    INTRODUCTION: Herpes simplex virus type 2 (HSV-2) is the most common cause of genital herpes. Glycoprotein G (gG) is a prototype antigen for type-specific serodiagnosis distinguishing between HSV type 1 (HSV-1) and HSV-2 infections. As immunological diagnosis kits for accurate differentiation between HSV-1 and HSV-2 antibodies can be expensive, there is a need to develop a convenient, sensitive, specific and cost-effective serodiagnostic kit. METHODS: We successfully expressed a fragment of gG comprising residues 321-580 of HSV-2 with histidine tag (gG(321-580His)) in a Bac-to-Bac baculovirus expression system, which had an antigenicity similar to its native counterpart. An indirect enzyme-linked immunosorbent assay (ELISA) was developed using gG(321-580His) as the diagnostic antigen and evaluated by comparison with a commercial HerpeSelect 2 ELISA immunoglobulin G kit as reference. RESULTS: In testing 318 field serum samples, the diagnostic relative sensitivity and specificity of the developed gG(321-580His)-ELISA test in qualitative comparison with the commercial kit were 93.81% and 96.74%, respectively, and the accuracy was 94.65%. CONCLUSION: The study indicates that gG(321-580His) has a high diagnostic potential for HSV-2 virus serodiagnosis in humans.
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