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  • Title: Characterization and homologous overexpression of an N-acetylglucosaminidase Nag1 from Trichoderma reesei.
    Author: Chen F, Chen XZ, Qin LN, Tao Y, Dong ZY.
    Journal: Biochem Biophys Res Commun; 2015 Apr 03; 459(2):184-188. PubMed ID: 25534854.
    Abstract:
    Trichoderma reesei is thought to be a promising recombinant host for the production and secretion of complex proteins due to its ability to secrete large amounts of proteins. In this study we identified a functional N-acetyl-β-glucosaminidase (NAGase) gene Nag1 in T. reesei. Nag1, a putative gene encoding a GH 20 family NAGase in T. reesei, was cloned and homologous overexpressed in the T. reesei RutC30ΔU3 with a strong cellobiohydrolase1 gene (cbh1) promoter. Nag1 was secreted in its active form and the highest expression level was around 499.85IU/ml. Nag1 has a molecular mass of 80kDa. The optimum pH and temperature were 4.0 and 60°C, respectively.
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