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  • Title: Characterization of rotavirus subgroup-specific monoclonal antibodies and use in single-sandwich ELISA systems for rapid subgrouping of human strains.
    Author: Gerna G, Sarasini A, Torsellini M, di Matteo A, Baldanti F, Parea M, Battaglia M.
    Journal: Arch Virol; 1989; 107(3-4):315-22. PubMed ID: 2554860.
    Abstract:
    Two subgroup-specific monoclonal antibodies (MAb) raised in mice against group A human rotavirus were shown to react by immunoblotting with the trimeric form of VP6 of the homologous subgroup and successfully applied to development of new single-sandwich ELISA systems for rapid subgrouping of human strains. All of the 344 strains tested could be subgrouped, but for two of them prior propagation in cell cultures was required.
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