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  • Title: Comprehensive NMR analysis of compositional changes of black garlic during thermal processing.
    Author: Liang T, Wei F, Lu Y, Kodani Y, Nakada M, Miyakawa T, Tanokura M.
    Journal: J Agric Food Chem; 2015 Jan 21; 63(2):683-91. PubMed ID: 25549134.
    Abstract:
    Black garlic is a processed food product obtained by subjecting whole raw garlic to thermal processing that causes chemical reactions, such as the Maillard reaction, which change the composition of the garlic. In this paper, we report a nuclear magnetic resonance (NMR)-based comprehensive analysis of raw garlic and black garlic extracts to determine the compositional changes resulting from thermal processing. (1)H NMR spectra with a detailed signal assignment showed that 38 components were altered by thermal processing of raw garlic. For example, the contents of 11 l-amino acids increased during the first step of thermal processing over 5 days and then decreased. Multivariate data analysis revealed changes in the contents of fructose, glucose, acetic acid, formic acid, pyroglutamic acid, cycloalliin, and 5-(hydroxymethyl)furfural (5-HMF). Our results provide comprehensive information on changes in NMR-detectable components during thermal processing of whole garlic.
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