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  • Title: Some physico-chemical properties of the rigid form of the Sendai virus nucleocapsid.
    Author: Repanovici R, Hristova M, Popa LM.
    Journal: Virologie; 1989; 40(3):189-96. PubMed ID: 2556836.
    Abstract:
    The effect of some dissociation agents (SDS, beta-mercaptoethanol, urea, EDTA) on the rigid form of the Sendai virus nucleocapsid was studied. Polyacrylamide gel electrophoresis in the presence of lytic mixture (1% SDS, 2% beta-mercaptoethanol, 5 M urea, for 2 min at 100 degrees C) revealed two types of polypeptide subunits (mol. wts. 46,000 and 14,000), as well as the dissociation in the presence of 0.1% SDS only. The EDTA treatment leads to a disorganization of the protein part (10(-2) M) or of the nucleocapsid structure (5 x 10(-2) M).
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