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  • Title: Synthesis of a red fluorescent dye-conjugated Ag@SiO2 nanocomposite for cell immunofluorescence.
    Author: Dong M, Tian Y, Pappas D.
    Journal: Appl Spectrosc; 2015; 69(2):215-21. PubMed ID: 25587713.
    Abstract:
    In this work we describe a one-step approach for incorporating a red fluorophore (2SBPO) into core-shell nanoparticles for metal-enhanced fluorescence immunolabels. The 2SBPO-MEF nanoparticles are particularly attractive as cell labels because their ∼ 670 nm emission has minimal overlap with cell autofluorescence and from overlap with many conventional probes. 2SBPO was incorporated through physical entrapment during the Stöber process. Antibody-based cell labels were then synthesized using covalent linkage. The nanoparticle fluorescence was 7.5-fold higher than control nanoparticles lacking a metal core. We demonstrated labeling of CD4 + HuT 78 T lymphocytes using anti-CD4-conjugated nanoparticle labels. Cells labeled with anti-CD4 nanoparticles showed a 35-fold fluorescence signal compared to anti-CD4 coreless controls. This simple synthesis protocol can be applied to a variety of hydrophilic fluorophore types and has broad potential in bioanalytical and biosensing applications.
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