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  • Title: Rapid detection of herpes simplex virus DNA by in situ hybridization with photobiotin-labelled double-stranded DNA probes.
    Author: Chantratita W, Henchal EA, Yoosook C.
    Journal: Mol Cell Probes; 1989 Dec; 3(4):363-73. PubMed ID: 2559320.
    Abstract:
    An assay for rapid detection of herpes simplex virus in infected cells is described. The assay utilizes in situ hybridization with photobiotin-labelled double-stranded DNA probes prepared from HSV-1 DNA cloned in plasmid vectors. The assay provided an alternative method for earlier detection of virus in cell cultures with the ease of preparation of photobiotin-labelled double-stranded DNA.
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