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  • Title: Characterization of human T-cell lines harboring defective human immunodeficiency virus type 1.
    Author: Hamamoto Y, Takamatsu K, Kobayashi S, Yamaguchi K, Yamamoto N, Kobayashi N.
    Journal: Virus Genes; 1989 Nov; 3(2):141-52. PubMed ID: 2559541.
    Abstract:
    Defective HIV-producing T-cell lines were subcloned from MT-4/HIVHTLV-IIIB' MOLT-4/HIVHTLV-IIIB, and H9/HIVHTLV-IIIB cell lines chronically infected with HIV. The NY-M10 cell line derived from MOLT-4/HIVHTLV-IIIB and the NY-H6 cell line derived from H9/HIVHTLV-IIIB produce defective HIV, which lacks the ability to infect human T-cell lines. NY-M10 cells retain the capacity to form multinucleated giant cells in cocultivation with HIV-uninfected CD4-positive cells. However, NY-H6 cells failed to fuse with CD4-positive cells. Electron microscopic analysis indicated that the defective HIV produced from NY-M10, like those reported previously, lacked the structure of the nucleocapsid, and the virion released from NY-H6 was indistinguishable from those of authentic HIV particles. Southern and Northern blotting analyses of NY-M10 and NY-H6 cleared that the genome of those defective viruses was not significantly deleted, suggesting minor mutation(s) should take place on the viral genome.
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