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Title: Axial ligand replacement in horse heart cytochrome c by semisynthesis. Author: Raphael AL, Gray HB. Journal: Proteins; 1989; 6(3):338-40. PubMed ID: 2560194. Abstract: Semisynthesis has been employed to replace the axial methionine in horse heart cytochrome c with histidine. The reduction potential of the His-80 protein (cyt c-His-80) is 41 mV vs NHE (0.1 M phosphate; pH 7.0; 25 degrees C). The absorption spectra of oxidized and reduced cyt c-His-80 are very similar to those of the native protein in the porphyrin region, but the 695 nm band is absent in the oxidized His-80 protein.[Abstract] [Full Text] [Related] [New Search]