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  • Title: Enzyme assay for pyridoxal 5'-phosphate by apo-D-amino acid aminotransferase.
    Author: Ota H, Mushiga M, Yoshimura T, Yoshimune K.
    Journal: J Biosci Bioeng; 2015 Jul; 120(1):117-9. PubMed ID: 25622769.
    Abstract:
    D-amino acid aminotransferase (D-AAT) is a pyridoxal 5'-phosphate (PLP)-dependent enzyme. D-AAT from thermophilic Bacillus sp. YM-1 was inactivated by ultraviolet irradiation. The activity was restored by the addition of PLP and showed a linear correlation with respect to PLP concentrations in the range of 10-40 nM.
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