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  • Title: Studies on the determination of Na-K-ATPase in red blood cell membranes.
    Author: Eder K, Reichlmayr-Lais AM, Kirchgessner M.
    Journal: J Trace Elem Electrolytes Health Dis; 1989 Sep; 3(3):151-9. PubMed ID: 2562499.
    Abstract:
    The present paper is concerned with Na-K-ATPase. In the first part it deals with the effect of such factors as Na+, K+, Mg2+, ATP, pH on activity investigated using a soluble Na-K-ATPase from porcine cerebral cortex. In the second part a method for assaying red blood cell Na-K-ATPase is described. This method consists of specific steps for washing and preparation of red cell membranes, pretreatment of Na-K-ATPase with sodium dodecyl sulfate (SDS) and bovine serum albumin (BSA) and determination of enzymatic released inorganic phosphate. Na-K-ATPase prepared and treated in this way shows a higher activity than the conventionally prepared form of enzyme, as latent enzyme sites are exposed by the SDS treatment. The kinetic behaviour of Na-K-ATPase prepared and assayed by using this method is characterized and compared with that of soluble Na-K-ATPase.
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