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  • Title: A sensor for quantification of macromolecular crowding in living cells.
    Author: Boersma AJ, Zuhorn IS, Poolman B.
    Journal: Nat Methods; 2015 Mar; 12(3):227-9, 1 p following 229. PubMed ID: 25643150.
    Abstract:
    Macromolecular crowding in cells influences processes such as folding, association and diffusion of proteins and polynucleic acids. Direct spatiotemporal readout of crowding would be a powerful approach for unraveling the structure of the cytoplasm and determining the impact of excluded volume on protein function in living cells. Here, we introduce a genetically encodable fluorescence resonance energy transfer (FRET) sensor for quantifying macromolecular crowding and discuss our application of the sensor in bacterial and mammalian cells.
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