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  • Title: [Antibacterial activity of synthetic antimicrobial decapeptide against oral bacteria].
    Author: Yi L, Wei F, Lina W, Guangyan D, Hongkun W.
    Journal: Hua Xi Kou Qiang Yi Xue Za Zhi; 2014 Dec; 32(6):601-5. PubMed ID: 25665430.
    Abstract:
    OBJECTIVE: This study aims to evaluate the antimicrobial activity of decapeptide, a novel antimicrobial peptide, against several major cariogenic and periodontopathogenic bacteria in vitro. METHODS In this study, we investigated the antimicrobial activity of decapeptide against Streptococcus mutans (S. mutans), Streptococcus sobrinus, Lactobacillus acidophilus, Streptococcus sanguis, Streptococcus gordonii, Actinomyces viscosus, Actinomyces naeslundii, Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans, and Saccharomyces albicans in vitro using the agar diffusion method and broth dilution method. Furthermore, a time-kill kinetic study of decapeptide against S. mutans was performed. RESULTS: The results showed that decapeptide exhibited antimicrobial activity against various oral bacteria and fungi. The minimum inhibitory concentration (MIC) of main cariogenic bacteria ranged from 62.5 μg · mL(-1) to 125 μg · mL(-1), and the MIC of periodontopathogenic bacteria tested ranged from 250 μg · mL(-1) to 1,000 μg · mL(-1). Among the bacteria tested, decapeptide had a strong inhibitory effect on cariogenic S. mutans. Results of the time-kill kinetic studies showed that decapeptide reduced the viable counts of S. mutans by more than one order of magnitude after 20 min of incubation, and thoroughly killed S. mutans after 30 min. No viable cells could be detected after 24 h of incubation. CONCLUSION: This study suggest that decapeptide might have potential clinical application in treating dental caries by killing S. mutans within dental plaque. 目的: 评价人工合成抗菌肽（十肽）对口腔常见感染性疾病主要致病菌的抑菌活性。 方法: 采用琼脂扩散法及液体稀释法体外评价十肽对变异链球菌、表兄链球菌、嗜酸乳杆菌、血链球菌、格氏链球菌、黏性放线菌、内氏放线菌、牙龈卟啉单胞菌、中间普雷沃菌、具核梭杆菌、伴放线嗜血杆菌及白色假丝酵母菌的抑菌性能，并测定十肽对变异链球菌的时间-杀菌曲线。 结果: 十肽对所选实验菌株均表现出不同的抑菌性能，对主要致龋菌的最小抑菌浓度MIC值为62.5~125 µg·mL−1，而对主要牙周致病菌的MIC值为250~1 000 µg·mL−1，其中，十肽对龋病主要致病菌变异链球菌有较强抑菌作用。时间-杀菌曲线结果显示，十肽作用20 min后开始杀菌，30 min之后可完全杀灭细菌，且在24 h之内无细菌生长。 结论: 新型人工合成抗菌肽十肽对口腔常见感染性疾病主要致病菌具有抑菌性能，其中对致龋变异链球菌的抑菌效果最为明显。
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