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  • Title: Serological diagnosis of salmonella infections by enzyme immunoassay.
    Author: Isomäki O, Vuento R, Granfors K.
    Journal: Lancet; 1989 Jun 24; 1(8652):1411-4. PubMed ID: 2567429.
    Abstract:
    An enzyme immunoassay (EIA) for the detection and measurement of serum IgM, IgG, and IgA antibodies to salmonella was developed with commercially available lipopolysaccharides (LPSs) of Salmonella typhimurium and S enteritidis combined as antigen. Of 130 sera from patients with culture-confirmed salmonella infections, 115 (88.5%) were positive in this assay. The classic Widal agglutination test was positive in only 50 (38.5%) cases. This EIA method offers a substantial advance in the serological diagnosis of acute salmonella infections; it detects antibodies to the salmonellae of groups B and D, which constitute about 70% of culture-positive cases of human salmonellosis. Antibodies to other salmonellae are also detected. This EIA is particularly valuable for the detection of salmonella antibodies during post-infectious complications when isolation of the organism is often no longer possible.
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