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  • Title: Rapid detection of ochratoxin A on membrane by dot immunogold filtration assay.
    Author: Chen W, Jin Y, Liu A, Wang X, Chen F.
    Journal: J Sci Food Agric; 2016 Jan 30; 96(2):610-4. PubMed ID: 25678129.
    Abstract:
    BACKGROUND: Ochratoxin A (OTA), a widely distributed mycotoxin produced by certain species of Aspergillus and Penicillium, has been identified as a carcinogenic, hepatotoxic, teratogenic, nephrotoxic and immunotoxic toxin. To reduce the risk of OTA contamination, a rapid, inexpensive, suitable and on-site assay for its detection is required. RESULTS: In this study a dot immunogold filtration assay (DIGFA) of OTA on high-flow nitrocellulose membrane was developed. Firstly colloidal gold was synthesized and colloidal gold-polyclonal antibody (PcAb) conjugates against OTA were prepared at the optimal colloidal gold-labeled pH value and package amount. Then the colloidal gold-PcAb conjugates were used to develop the OTA DIGFA. The results demonstrated a visual detection limit of approximately 10 ng mL(-1) OTA. In addition, this method had no cross-reaction with zearalenone, aflatoxin B1 or citrinin. CONCLUSION: These results indicated that the developed DIGFA could be applied for the actual detection of samples without complicated steps.
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