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  • Title: Fluorescence-based detection of single-nucleotide changes in RNA using graphene oxide and DNAzyme.
    Author: Hong C, Kim DM, Baek A, Chung H, Jung W, Kim DE.
    Journal: Chem Commun (Camb); 2015 Apr 04; 51(26):5641-4. PubMed ID: 25714982.
    Abstract:
    We report a simple fluorometric method for detection of single-nucleotide changes in RNA using graphene oxide (GO) and RNA-cleaving DNAzyme. The fluorescent DNA probe (F-DNA) was annealed to RNA fragments generated by RNA cleavage with DNAzyme specific to mutant RNA. The F-DNA-RNA duplex attenuated the quenching of F-DNA fluorescence by GO.
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