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  • Title: Measurement of microcystin -LR in water samples using improved HPLC method.
    Author: Shamsollahi HR, Alimohammadi M, Nabizadeh R, Nazmara S, Mahvi AH.
    Journal: Glob J Health Sci; 2014 Sep 28; 7(2):66-70. PubMed ID: 25716387.
    Abstract:
    Microcystins are a group of toxic compounds produced by freshwater cyanobacteria and cause diseases. World Health Organization has recommended a concentration of 1 µg/l for Microcystin-LR (MC-LR) in potable water as guideline value. The high performance liquid chromatography (HPLC) followed by C18 analytical column and ultra violet detector for detection of MC-LR. In this regard, 5 different concentrations of MC-LR solutions were injected into HPLC. MC-LR was detected in 5.33 minute retention time and Calibration curve was achieved with R(2) = 0.988. Detection limit for this method was obtained by using acetonitrile solutions (32% and 55%) as a gradient run and a high silanol activity column equal to 0.02 µg /mL. Despite no acidic organic modifier being used in the mixture of solvents, the sensitivity of this method was appropriate for detection of MC-LR. Because of short retention time, reduction in number of solvents and high resolution and suitable sensitivity, this method is affordable and is fast for detection and determination of MC-LR in potable water.
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