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  • Title: Investigation of the binding between pepsin and nucleoside analogs by spectroscopy and molecular simulation.
    Author: Li Z, Li Z, Yang L, Xie Y, Shi J, Wang R, Chang J.
    Journal: J Fluoresc; 2015 Mar; 25(2):451-63. PubMed ID: 25721993.
    Abstract:
    In this paper, the interactions of pepsin with CYD (cytidine) or nucleoside analogs, including FNC (2'-deoxy-2'-β-fluoro-4'-azidocytidine) and CMP (cytidine monophosphate), were investigated by fluorescence, UV-visible absorption and synchronous fluorescence spectroscopy under mimic physiological conditions. The results indicated that FNC (CYD/CMP) caused the fluorescence quenching by the formation of complex. The binding constants and thermo-dynamic parameters at three different temperatures were obtained. The hydrophobic and electrostatic interactions were the predominant intermolecular forces to stabilize the complex. The F atom in FNC might weaken the binding of nucleoside analog to pepsin. Results showed that CYD was the strongest quencher and bound to pepsin with higher affinity.
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