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  • Title: Development of a hybridoma cell line secreting monoclonal antibody against S protein of a Chinese variant of PEDV.
    Author: Lei X, Zhao J, Wang X, Zhao Y, Wang C.
    Journal: Monoclon Antib Immunodiagn Immunother; 2015 Feb; 34(1):12-6. PubMed ID: 25723278.
    Abstract:
    Gene encoding 22-380 aa of spike protein of porcine epidemic diarrhea virus (PEDV) isolate in China was cloned and expressed as a recombinant protein in Escherichia coli BL21(DE3). Female BALB/c mice were immunized with the purified recombinant S protein, and a monoclonal antibody (MAb) designated as 2D1 against S protein was achieved by hybridoma technique. MAb 2D1 reacted with S protein of PEDV specifically. The monoclonal antibody 2D1 may provide a useful tool as a specific diagnostic reagent for detecting S protein of the Chinese variant of PEDV and for further investigation into the virus' pathogenic mechanism.
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