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  • Title: Enzymatic biodiesel synthesis from yeast oil using immobilized recombinant Rhizopus oryzae lipase.
    Author: Duarte SH, Hernández GL, Canet A, Benaiges MD, Maugeri F, Valero F.
    Journal: Bioresour Technol; 2015 May; 183():175-80. PubMed ID: 25731926.
    Abstract:
    The recombinant Rhizopus oryzae lipase (1-3 positional selective), immobilized on Relizyme OD403, has been applied to the production of biodiesel using single cell oil from Candida sp. LEB-M3 growing on glycerol from biodiesel process. The composition of microbial oil is quite similar in terms of saponifiable lipids than olive oil, although with a higher amount of saturated fatty acids. The reaction was carried out in a solvent system, and n-hexane showed the best performance in terms of yield and easy recovery. The strategy selected for acyl acceptor addition was a stepwise methanol addition using crude and neutralized single cell oil, olive oil and oleic acid as substrates. A FAMEs yield of 40.6% was obtained with microbial oils lower than olive oil 54.3%. Finally in terms of stability, only a lost about 30% after 6 reutilizations were achieved.
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