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  • Title: Preparation, characterization, and antibacterial activity of silver nanoparticle-decorated graphene oxide nanocomposite.
    Author: Shao W, Liu X, Min H, Dong G, Feng Q, Zuo S.
    Journal: ACS Appl Mater Interfaces; 2015 Apr 01; 7(12):6966-73. PubMed ID: 25762191.
    Abstract:
    In this work, we report a facile and green approach to prepare a uniform silver nanoparticles (AgNPs) decorated graphene oxide (GO) nanocomposite (GO-Ag). The nanocomposite was fully characterized by transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectra, ultraviolet-visible (UV-vis) absorption spectra, and X-ray photoelectron spectroscopy (XPS), which demonstrated that AgNPs with a diameter of approximately 22 nm were uniformly and compactly deposited on GO. To investigate the silver ion release behaviors, HEPES buffers with different pH (5.5, 7, and 8.5) were selected and the mechanism of release actions was discussed in detail. The cytotoxicity of GO-Ag nanocomposite was also studied using HEK 293 cells. GO-Ag nanocomposite displayed good cytocompatibility. Furthermore, the antibacterial properties of GO-Ag nanocomposite were studied using Gram-negative E. coli ATCC 25922 and Gram-positive S. aureus ATCC 6538 by both the plate count method and disk diffusion method. The nanocomposite showed excellent antibacterial activity. These results demonstrated that GO-Ag nanocomposite, as a kind of antibacterial material, had a great promise for application in a wide range of biomedical applications.
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