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  • Title: In situ detection of DNA-binding proteins in herpes simplex virus type 1-infected cells.
    Author: Mohammad F, Farber E.
    Journal: Acta Virol; 1989 Sep; 33(5):401-9. PubMed ID: 2576580.
    Abstract:
    An in situ assay for detecting DNA-binding proteins in herpes simplex virus type 1 (HSV-1)-infected cells is described. Seventeen HSV-induced DNA-binding species were visible with nicked, double-stranded DNA as a substrate, while fourteen virus-induced DNA-binding fractions were present in gels containing nuclease-treated, single-stranded DNA. The effects of HSV on cellular DNA-binding protein expression could also be seen. The resolution of DNA-binding fractions was dependent upon the type of DNA substrate utilized, high salt extraction of DNA-binding components and their physical separation from infected cell DNAs, dialysis of the high salt and the length of DNase treatment of gels following electrophoresis.
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