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  • Title: High resolution R banding in amniotic fluid cells using the BrdU-Hoechst 33258-Giemsa (RBG) technique.
    Author: Cheung SW, Crane JP, Burgess AC.
    Journal: Hum Genet; 1985; 69(1):86-7. PubMed ID: 2578426.
    Abstract:
    While standard Giemsa banding is generally adequate for amniotic fluid chromosome analysis, small deletions, duplications, or translocation breakpoints involving G-negative bands may be difficult to appreciate. We report a method for producing high resolution R banding in human amniotic fluid cultures using the BrdU-Hoechst 33258-Giemsa (RBG) technique. RBG banding can be useful in confirming and precisely defining structural abnormalities in amniotic fluid cultures.
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