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  • Title: The measurement of cardiac membrane channels following their incorporation into phospholipid bilayers.
    Author: Williams AJ.
    Journal: Adv Myocardiol; 1985; 5():77-84. PubMed ID: 2578684.
    Abstract:
    This chapter describes the basic properties of integral membrane channels from both cardiac sarcoplasmic reticulum and sarcolemma. Channels are studied, under voltage-clamp conditions, following their incorporation into planar phospholipid bilayers by fusion of isolated native membrane vesicles with preformed membranes. The rate of fusion of vesicles may be influenced by a number of factors including divalent cations and negatively charged phospholipids in the preformed bilayer. Mammalian cardiac sarcoplasmic reticulum contains a monovalent cation selective channel with a single-channel conductance of approximately 150 pico-Siemens in the presence of symmetrical solutions of 500 mM K+ at holding potentials ranging from -60 to +60 mV. The probability of the channel being in the open state is high at positive holding potentials and low at negative holding potentials. Mammalian cardiac sarcolemma contains at least three K+-selective channels and one Cl(-)-selective channel.
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