These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


PUBMED FOR HANDHELDS

Search MEDLINE/PubMed


  • Title: A simple HPLC-UV method for the determination of ciprofloxacin in human plasma.
    Author: Vella J, Busuttil F, Bartolo NS, Sammut C, Ferrito V, Serracino-Inglott A, Azzopardi LM, LaFerla G.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2015 May 01; 989():80-5. PubMed ID: 25813900.
    Abstract:
    A rapid and sensitive HPLC-UV method for the determination of ciprofloxacin in human plasma is described. Protein precipitation with acetonitrile was used to separate the drug from plasma protein. An ACE(®) 5 C18 column (250 mm×4.6 mm, 5 μm) with an isocratic mobile phase consisting of phosphate buffer (pH 2.7) and acetonitrile (77:23, v/v) was used for separation. The UV detector was set at 277 nm. The method was validated in the linear range of 0.05-8 μg/ml with acceptable inter- and intra-assay precision, accuracy and stability. The method is simple and rapid and can be used to quantify this widely used antibiotic in the plasma of patients suffering from Peripheral Arterial Disease.
    [Abstract] [Full Text] [Related] [New Search]