These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Blot analyses of glycoconjugates: enzyme-hydrazide--a novel reagent for the detection of aldehydes.
    Author: Gershoni JM, Bayer EA, Wilchek M.
    Journal: Anal Biochem; 1985 Apr; 146(1):59-63. PubMed ID: 2581474.
    Abstract:
    A procedure for the general staining of glycoproteins and other glycoconjugates on protein blots has been developed. Aldehydes are formed on the sugars of glycoconjugates by periodate oxidation which then react with hydrazide groups of enzyme-hydrazides, a novel reagent designed for aldehyde detection. The bound enzyme-hydrazide is demonstrated histochemically. The new assay is advantageous over periodic-acid Schiff staining of gels as its reagents and signals are stable and the process is simple and expedient, and provides greater sensitivity.
    [Abstract] [Full Text] [Related] [New Search]