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  • Title: [Human T-lymphocytes--clinical and methodological studies on the detection of Fc-IgM- and Fc-IgG-receptor bearing T-lymphocytes (Tm and Tg)].
    Author: Grunow G, Volk HD, Ittenson A.
    Journal: Folia Haematol Int Mag Klin Morphol Blutforsch; 1985; 112(1):109-24. PubMed ID: 2581856.
    Abstract:
    Several methodical aspects for determination of T lymphocytes with Fc receptors for IgM (TM) and IgG (TG) were studied including separation technique of T cells with E-rosetting, culture conditions of T cells for determination of TM and the rosetting of TM and TG with EA complexes. The bests results were obtained by stabilization of E-rosettes with human serum albumin, after separation of E-rosette forming cells lysis of sheep erythrocytes with save hypotonic shock, culturing of T cells in medium containing 20% AB Serum. Furthermore it was shown the possibility using EA complexes produced with not purifieded IgG or IgM anti-ox-red-blood cells antisera without lost of specifity for TM and TG. The percentage of TM and TG in peripheral blood of thirty healthy persons as well as monitoring TM and TG in three cases was investigated.
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