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  • Title: Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides.
    Author: Kempe T, Sundquist WI, Chow F, Hu SL.
    Journal: Nucleic Acids Res; 1985 Jan 11; 13(1):45-57. PubMed ID: 2582352.
    Abstract:
    Biotin has been converted to 2-(biotinylamido)ethanol and condensed to phosphorylated oligonucleotides in a solid phase synthesis. The 5'-biotinylated oligonucleotides were enzymatically coupled to other DNA fragments by T4 DNA ligase or T4 RNA ligase. The hybridization properties of such biotin-labeled oligonucleotide probes were studied.
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