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  • Title: Activin A can induce definitive endoderm differentiation from human parthenogenetic embryonic stem cells.
    Author: Wang Z, Li W, Chen T, Yang J, Wen Z, Yan X, Shen T, Liang R.
    Journal: Biotechnol Lett; 2015 Aug; 37(8):1711-7. PubMed ID: 25851951.
    Abstract:
    OBJECTIVES: As activin/nodal signaling plays a key role in definitive endoderm (DE) differentiation, we have explored activin A-induced differentiation of DE from human parthenogenetic embryonic stem cells (hPESCs). RESULTS: Administration of 5 ng activin A/ml had no effect on the expression of markers of DE differentiation. However, higher concentrations of activin A (50 and 100 ng/ml) upregulated Sox17 and Cxcr4, as well upregulating the mesendodermal precursor marker, Brachyury. CONCLUSIONS: These findings demonstrate that low dose activin A can maintain the undifferentiated potency of hPESCs, whereas higher doses induce DE differentiation; 50 ng/ml is the optimal concentration for inducing DE from hPESCs.
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