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  • Title: [Kinetic characteristics of 6-phosphogluconate dehydrogenase from bull adrenal cortex].
    Author: Senkevich SB, Martynchik DI, Vinogradov VV.
    Journal: Ukr Biokhim Zh (1978); 1989; 61(5):92-5. PubMed ID: 2588353.
    Abstract:
    The hyperbolic dependence of the initial rate of 6-phosphogluconate dehydrogenase-catalyzed reaction on 6-phosphogluconate and NADP concentrations has been established. The Lineweaver-Burk plots of V0 against concentration of one substrate with constant unsaturating concentrations of another substrate cross left from the ordinate axis. The Km value for 6-phosphogluconate is equal ot 0.035 mM, for NADP--0.018 mM. It has been shown that NADPH inhibits 6-phosphogluconate dehydrogenase by the competitive type with respect to NADP and by the noncompetitive one with respect to 6-phosphogluconate. Ribulose-5-phosphate inhibits the reaction by the mixed type with respect to NADP and by the noncompetitive type with respect to 6-phosphogluconate. Kinetic data are in agreement with the consecutive mechanism of the reaction: the first substrate is NADP, the last product--NADPH. The Arrhenius plot for the reaction shows a break at 27 degrees C.
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