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  • Title: Effects of manipulating cyclic duration and pH on fermentative hydrogen production in an anaerobic sequencing batch reactor.
    Author: Won SG, Lau AK.
    Journal: J Environ Sci Health A Tox Hazard Subst Environ Eng; 2015; 50(7):750-6. PubMed ID: 25901853.
    Abstract:
    The effects of cyclic duration and pH on biological hydrogen production were investigated in an anaerobic sequencing batch reactor. Experiments were conducted using cyclic duration of (4, 8, and 12 h) in combination with pH (4, 5, and 6) in a 3 × 3 factorial design, while hydraulic retention time and organic loading rate were maintained at 24 h and 10.3 g COD L(-1).d(-1), respectively. At pH 4, the effect of cyclic duration on hydrogen production was found to be insignificant. However, in runs with pH 5 and 6, a shorter cyclic duration of 4 h led to lower hydrogen productivity. The operational condition (pH 6, cyclic duration 12 h) induced higher hydrogen production rate of 2.3 ± 0.6 L H2/L reactor.d, whereas higher hydrogen yield of 2.2 ± 0.4 mol H2/mol sucrose was achieved at pH 5 and the same 12 h cyclic duration. The differences in hydrogen production were not statistically significant between 8 h and 12 h cyclic duration. Higher hydrogen production rates were associated with biomass (mixed liquor volatile suspended solids) concentration ranging from 8-13 g L(-1), but further increase in biomass growth was not accompanied by increased hydrogen production. Furthermore, a food-to-microorganism ratio of 0.84 was found to result in higher hydrogen production rate.
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