These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Determination of phenol in water by electrochemical tyrosinase biosensor based on ordered graphitized mesoporous carbon and evaluated by high performance liquid chromatography].
    Author: Wu L, Liu H, Li J, Fu X, Song Y.
    Journal: Se Pu; 2014 Dec; 32(12):1368-72. PubMed ID: 25902645.
    Abstract:
    A novel electrochemical tyrosinase biosensor based on ordered graphitized mesoporous carbon (GMC) was obtained, which was used as a platform for phenol detection. The accuracy of tyrosinase biosensor method was comparatively evaluated by high performance liquid chromatography (HPLC). By entrapping tyrosinase molecules (6.5 nm x 9.8 nm x 5.5 nm) into the mesopores of GMC (diameter 10 nm, GMC10), the "interspace confinement effect" of GMC10 may improve the stability of tyrosinase in vitro. After 21-day storage, the GMC10-based tyrosinase biosensor retained more than 85% of its initial response. It is indicated that GMC10 with "interspace confinement effect" can significantly prolong the life of tyrosinase molecules in vitro. Furthermore, the GMC-based tyrosinase biosensor displayed excellent analytical performances for phenol detection, such as stability, repeatability, selectivity, sensitivity and limit of detection. The GMC-based tyrosinase biosensor demonstrated a linear response for phenol from 0. 1 to 10 µmol/L with a low detection limit of 20 nmol/L. The comparative study between HPLC and GMC-based tyrosinase biosensor showed that the detection of phenol in water sample by the GMC-based tyrosinase biosensor method is reliable, accurate and effective. The proposed GMC-based tyrosinase biosensor proved to be a very promising "pre-alarm" tool for rapid detecting phenol pollution in emergency accidents.
    [Abstract] [Full Text] [Related] [New Search]