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  • Title: [Expression of inulinase genes in the yeasts Saccharomyces cerevisiae and Kluyveromyces marxianus].
    Author: Sokolenko GG, Karpechenko NA.
    Journal: Mikrobiologiia; 2015; 84(1):37-42. PubMed ID: 25916145.
    Abstract:
    Expression of the genes encoding the enzymes involved in inulin, sucrose, and glucose metabolism in the yeasts Saccharomyces cerevisiae and Kluyveromyces marxianus was studied. The exon-intron structure of the relevant genes was identified and the primers for quantitative PCR were optimized. Expression of the genes was found to depend on the carbon source. Glucose was shown to exhibit a repressive effect on inulinase synthesis by K. marxianus, while in S. cerevisiae glucose and sucrose were inulinase inducer and repressor, respectively.
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