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  • Title: Highly sensitive fluorescent stain for detecting lipopolysaccharides in sodium dodecyl sulfate polyacrylamide gel electrophoresis.
    Author: Wang X, Zhou A, Cai W, Yu D, Zhu Z, Jiang C, Jin L.
    Journal: Electrophoresis; 2015 Aug; 36(15):1795-800. PubMed ID: 25930092.
    Abstract:
    A sensitive and simple technique was developed for the visualization of gel-separated lipopolysaccharides by using a hydrazide derivative, UGF202. As low as 0.5-1 ng total LPS could be detected by UGF202 stain, which is 2- and 16-fold more sensitive than that of the commonly used Pro-Q Emerald 300 and Keenan et al. developed silver stain, respectively. The results indicated that UGF202 stain could be a good choice for LPS determination in polyacrylamide gels.
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