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  • Title: Solvent systems for thin layer chromatography of biological dyes.
    Author: Allison RT, Garratt NJ.
    Journal: Med Lab Sci; 1989 Apr; 46(2):113-9. PubMed ID: 2593771.
    Abstract:
    Thirty-nine dyes were subjected to thin layer chromatography to evaluate eight different solvent systems. The results obtained were analysed by dye class following assessment in daylight and ultra violet light. N-butanol: acetic acid: water (40:10:50) gave optimum separations of dyes viewed in visible light. Although n-butanol: ethanol: water (90:10:10) gave slightly better results for fluorescing compounds, material was frequently left at the start point with this solvent.
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