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  • Title: First Molecular Characterization of Cryptosporidium spp. Infecting Buffalo Calves in Brazil.
    Author: Aquino MC, Widmer G, Zucatto AS, Viol MA, Inácio SV, Nakamura AA, Coelho WM, Perri SH, Meireles MV, Bresciani KD.
    Journal: J Eukaryot Microbiol; 2015; 62(5):657-61. PubMed ID: 25941018.
    Abstract:
    With the aim of determining the occurrence of Cryptosporidium spp., 222 fecal samples were collected from Murrah buffalo calves aged up to 6 mo. Fecal DNA was genotyped with a nested polymerase chain reaction targeting the 18S rRNA gene and sequencing of the amplified fragment. Nested 18S PCR was positive for 48.2% of the samples. Sequence analysis showed that the most frequent species in these animals was Cryptosporidium ryanae, which was present in buffalo calves as young as 5 d. The zoonotic species Cryptosporidium parvum was detected in one animal. An uncommon Cryptosporidium 18S genotype was found in buffaloes.
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