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  • Title: Improvement of 1,3-propanediol production in Klebsiella pneumoniae by moderate expression of puuC (encoding an aldehyde dehydrogenase).
    Author: Zhu C, Jiang X, Zhang Y, Lin J, Fu S, Gong H.
    Journal: Biotechnol Lett; 2015 Sep; 37(9):1783-90. PubMed ID: 25957564.
    Abstract:
    OBJECTIVE: To improve 1,3-propanediol production in Klebsiella pneumoniae, the effects of puuC expression in lactate- and lactate/2,3-butanediol-deficient strains were assessed. RESULTS: Overexpression of puuC (encoding an aldehyde dehydrogenase) inhibited 1,3-propanediol production and increased 3-hydroxypropionic acid formation in both lactate- and lactate/2,3-butanediol-deficient strains. An improvement in 1,3-propanediol production was only achieved in a lactate-deficient strain via moderate expression of puuC; at the end of the fermentation, 1,3-propanediol productivity increased by 14% compared with the control. Further comparative analysis of the metabolic flux distributions in different strains indicated that 3-hydroxypropionic acid formation could play a considerable role in cell metabolism in K. pneumoniae. CONCLUSION: An improvement in 3-hydroxypropionic acid formation would be beneficial for cell metabolism, which can be accomplished by enhancing 1,3-propanediol productivity in a lactate-deficient strain via moderate expression of puuC.
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