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  • Title: A presequence-binding groove in Tom70 supports import of Mdl1 into mitochondria.
    Author: Melin J, Kilisch M, Neumann P, Lytovchenko O, Gomkale R, Schendzielorz A, Schmidt B, Liepold T, Ficner R, Jahn O, Rehling P, Schulz C.
    Journal: Biochim Biophys Acta; 2015 Aug; 1853(8):1850-9. PubMed ID: 25958336.
    Abstract:
    The translocase of the outer mitochondrial membrane (TOM complex) is the general entry gate into mitochondria for almost all imported proteins. A variety of specific receptors allow the TOM complex to recognize targeting signals of various precursor proteins that are transported along different import pathways. Aside from the well-characterized presequence receptors Tom20 and Tom22 a third TOM receptor, Tom70, binds proteins of the carrier family containing multiple transmembrane segments. Here we demonstrate that Tom70 directly binds to presequence peptides using a dedicated groove. A single point mutation in the cavity of this pocket (M551R) reduces the presequence binding affinity of Tom70 ten-fold and selectively impairs import of the presequence-containing precursor Mdl1 but not the ADP/ATP carrier (AAC). Hence Tom70 contributes to the presequence import pathway by recognition of the targeting signal of the Mdl1 precursor.
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