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  • Title: Laser fluorometric detection of porphyrin methyl esters for high-performance thin-layer chromatography.
    Author: Huie CW, Williams WR.
    Journal: Anal Chem; 1989 Oct 15; 61(20):2288-92. PubMed ID: 2596698.
    Abstract:
    A new detection method is presented for the determination of porphyrins present in biological materials. Separation is accomplished by high-performance thin-layer chromatography after esterification of individual carboxylic acid porphyrins. Detection is achieved by utilizing one of the visible lines of an argon-ion laser for fluorometric excitation. Good selectivity and detectability are demonstrated for the determination of porphyrin profiles in human urine. The detection limits for uro-, heptacarboxy-, hexacarboxy-, pentacarboxy-, copro-, and mesoporphyrin methyl esters are in the 18-35 pg range.
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