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  • Title: Nitrifying biomass characterization and monitoring during bioaugmentation in a membrane bioreactor.
    Author: D'Anteo S, Mannucci A, Meliani M, Verni F, Petroni G, Munz G, Lubello C, Mori G, Vannini C.
    Journal: Environ Technol; 2015; 36(24):3159-66. PubMed ID: 26017932.
    Abstract:
    A membrane bioreactor (MBR), fed with domestic wastewater, was bioaugmented with nitrifying biomass selected in a side-stream MBR fed with a synthetic high nitrogen-loaded influent. Microbial communities evolution was monitored and comparatively analysed through an extensive bio-molecular investigation (16S rRNA gene library construction and terminal-restriction fragment length polymorphism techniques) followed by statistical analyses. As expected, a highly specialized nitrifying biomass was selected in the side-stream reactor fed with high-strength ammonia synthetic wastewater. The bioaugmentation process caused an increase of nitrifying bacteria of the genera Nitrosomonas (up to more than 30%) and Nitrobacter in the inoculated MBR reactor. The overall structure of the microbial community changed in the mainstream MBR as a result of bioaugmentation. The effect of bioaugmentation in the shift of the microbial community was also verified through statistical analysis.
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