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  • Title: Extraction of tyrosine aminotransferase mRNA by polyribosome immunosorption on sepharose 4B.
    Author: Adler VV, Mechitov VI.
    Journal: Mol Biol (Mosk); 1978; 12(2):260-6. PubMed ID: 26021.
    Abstract:
    Sepharose 4B column with antibody to tyrosine aminotransferase (E.C. 2.6.1.5) (TAT) covalently bound can selectively remove a specific fraction of TAT polysomes from rat liver homogenates. From the polysomes which was adsorbed by immunosorbent one may obtain mRNA containing a segment of poly-adenilate-rich residues, having sedimentation constant of 11--16S. This mRNA may program synthesis of the specific protein in a cell free system. Near 70% of the protein was synthesized in such a system, may react with the antibody to TAT.
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